Oxidative stress impact on growth hormone secretion in the eye.

Šarić, Borna and Brzović Šarić, Vlatka and Barberić, Monika and Predović, Jurica and Rumenjak, Vlatko and Cerovski, Branimir (2015) Oxidative stress impact on growth hormone secretion in the eye. Croatian Medical Journal, 56 (4). pp. 326-33. ISSN 0353-9504

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Abstract

AIM: To evaluate the influence of oxidative stress on extrapituitary growth hormone (GH) secretion in the eye and to analyze the interdependence between eye and serum GH levels under normal and hypoxic conditions. ----- METHODS: Pars plana vitrectomy (PPV) was performed in 32 patients with developed proliferative diabetic retinopathy (PDR) and 49 non-diabetic controls, both of whom required this procedure as part of their regular treatment in the period from April 2013 to December 2014. During PPV, vitreous samples were taken and blood was simultaneously collected from the cubital vein. GH levels in serum and vitreous samples were measured by electrochemical luminescence assay. Oxidative stress was measured by enzyme-linked immunosorbent assay of advanced oxidation protein products (AOPP) and lipid hydroperoxide (LPO) in serum and vitreous. ----- RESULTS: Serum AOPP levels were significantly higher than vitreous levels in both groups (P<0.001 for each group) and LPO levels were significantly higher only in PDR group (P<0.001). There was a significant positive correlation between serum and vitreous LPO levels in PDR group (r=0.909; P<0.001). Serum GH levels were significantly higher than vitreous levels in both groups (P<0.001 for each group). Serum GH levels were significantly higher in PDR group than in controls (P=0.012). Vitreous GH values were slightly higher in PDR group, but the difference was not significant. ----- CONCLUSION: Our study confirms that GH production in the eye is autonomous and independent of oxidative stress or pituitary GH influence.

Abstract in Croatian

Utjecaj oksidacijskog stresa na sekreciju hormona rasta u oku ----- Cilj: Odrediti utjecaj oksidacijskog stresa na sekreciju ekstrapituitarnog hormona rasta u oku i analizirati međuovisnost razina hormona rasta u oku i serumu pod normalnim i pod hipotoksičnim uvjetima. ----- Postupci: 32 pacijenta s razvijenom proliferativnom dijabetičkom retinopatijom (PDR) i 49 kontrolnih ispitanika bez dijabetesa podvrgnuti su pars plana vitrektomiji (PPV) u razdoblju između travnja i prosinca 2013. U obje skupine ovaj postupak bio je dio redovnog liječenja. Za vrijeme zahvata uzeti su uzorci staklovine i istodobno je prikupljena krv iz kubitalne vene. Razine hormona rasta u staklovini i serumu mjerene su elektrokemijskom luminiscencijom. Oksidacijski stres mjeren je enzimskim imunotestom proizvoda unaprijeđene oksidacije proteina (eng, AOPP) i lipid hidroperoksida (LPO) u serumu i staklovini. ----- Rezultati: Razine AOPP-a u serumu bile su značajno više nego u staklovini u obje skupine (P<0,001 za svaku skupinu), dok su razine LPO bile značajno više samo u PDR skupini (P<0,001). Nije bilo značajne pozitivne korelacije između razina LPO u serumu i staklovini u PDR skupini (r=0,909; P<0,001). Razine hormona rasta u serumu su bile značajno više nego u staklovini u obje skupine (P<0,001 za svaku skupinu). Razine hormona rasta u serumu bile su značajno više u PDR skupini nego u kontrolnoj skupini (P=0,012). Razine hormona rasta u staklovini bile su nešto više u PDR skupini, ali razlika nije bila značajna. ----- Zaključak: Naše istraživanje potvrđuje da je sekrecija hormona rasta u oku autonomna i neovisi o oksidacijskom stresu ili utjecaju pituitarnog hormona rasta.

Item Type: Article
Departments: Katedra za oftalmologiju
Depositing User: Ana Babić
Status: Published
Creators:
CreatorsEmail
Šarić, BornaUNSPECIFIED
Brzović Šarić, VlatkaUNSPECIFIED
Barberić, MonikaUNSPECIFIED
Predović, JuricaUNSPECIFIED
Rumenjak, VlatkoUNSPECIFIED
Cerovski, BranimirUNSPECIFIED
Date: August 2015
Date Deposited: 23 Feb 2016 17:16
Last Modified: 23 Feb 2016 17:16
Subjects: /
Related URLs:
URI: http://medlib.mef.hr/id/eprint/2487

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