Hudolin, Tvrtko and Kaštelan, Željko and Ilić, Ivana and Levarda-Hudolin, Katarina and Bašić-Jukić, Nikolina and Rieken, Malte and Spagnoli, Giulio C. and Juretić, Antonio and Mengus, Chantal (2013) Immunohistochemical analysis of the expression of MAGE-A and NY-ESO-1 cancer/testis antigens in diffuse large B-cell testicular lymphoma. Journal of Translational Medicine, 11. p. 123. ISSN 1479-5876
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Abstract
BACKGROUND: Primary testicular lymphoma (PTL) is a rare and lethal disease. The most common histological subtype is diffuse large B-cell lymphoma (DLBCL). Standard treatments are frequently ineffective. Thus, the development of novel forms of therapy is urgently required. Specific immunotherapy generating immune responses directed against antigen predominantly expressed by cancer cells such as cancer-testis antigens (CTA) may provide a valid alternative treatment for patients bearing PTL, alone or in combination with current therapies. ----- METHODS: Three monoclonal antibodies (mAbs), 77B recognizing MAGE-A1, 57B recognizing an epitope shared by multiple MAGE-A CTA (multi-MAGE-A specific) and D8.38 recognizing NY-ESO-1/LAGE-1 were used for immunohistochemical staining of 27 PTL, including 24 DLBCL. ----- RESULTS: Expression of MAGE-A1 was infrequently detectable in DLBCL specimens (12.50%), whereas multi-MAGE-A and NY-ESO-1/LAGE-1 specific reagents stained the cytoplasms of tumor cells in DLBCL specimens with higher frequencies (54.17% and 37.50%, respectively) with different expression levels. ----- CONCLUSIONS: These results suggest that MAGE-A and NY-ESO-1/LAGE-1, possibly in combination with other CTA, might be used as targets for specific immunotherapy in DLBCL.
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| Additional Information: | © 2013 Hudolin et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | ||||||||||||||||||||
| MeSH: | Antibodies, Monoclonal/chemistry ; Antigens, Neoplasm/metabolism ; Cytoplasm/metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Immunohistochemistry ; Immunotherapy ; Lymphoma, B-Cell/metabolism ; Lymphoma, Large B-Cell, Diffuse/metabolism ; Male ; Melanoma-Specific Antigens/metabolism ; Membrane Proteins/metabolism ; Testicular Neoplasms/metabolism ; Testis/metabolism | ||||||||||||||||||||
| Departments: | Katedra za radiologiju i opću kliničku onkologiju Katedra za urologiju |
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| Depositing User: | Marijan Šember | ||||||||||||||||||||
| Status: | Published | ||||||||||||||||||||
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| Date: | 16 May 2013 | ||||||||||||||||||||
| Date Deposited: | 03 Feb 2016 09:28 | ||||||||||||||||||||
| Last Modified: | 13 Jul 2020 09:08 | ||||||||||||||||||||
| Subjects: | / | ||||||||||||||||||||
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| URI: | http://medlib.mef.hr/id/eprint/2393 |
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