Chemotactic and immunoregulatory properties of bone cells are modulated by endotoxin-stimulated lymphocytes

Cvija, Hrvoje and Kovačić, Nataša and Katavić, Vedran and Ivčević, Sanja and Aguila, Hector Leonardo and Marušić, Ana and Grčević, Danka (2012) Chemotactic and immunoregulatory properties of bone cells are modulated by endotoxin-stimulated lymphocytes. Inflammation, 35 (5). pp. 1618-1631. ISSN 0360-3997

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In our study, we explored the bidirectional communication via soluble factors between bone cells and endotoxin-stimulated splenic lymphocytes in an in vitro coculture model that mimics the inflammatory environment. Both the ability of lymphocytes to affect differentiation and immune properties of bone cells, osteoblasts (OBL) and osteoclasts (OCL), and of bone cells to modulate cytokine and activation profile of endotoxin-stimulated lymphocytes were tested. LPS-pulsed lymphocytes enhanced OCL but inhibited OBL differentiation and increased the RANKL/OPG ratio, and, at the same time, upregulated chemotactic properties of bone cells, specifically CCL2, CCL5, and CXCL10 in OCL and CCL5 and CXCL13 in OBL. In parallel, bone cells had immunosuppressive effects by downregulating the lymphocyte expression of interleukin (IL)-1, IL-6, TNF-α and co-stimulatory molecules. OCL stimulated the production of osteoclastogenic cytokine RANKL in T lymphocytes. The anti-inflammatory effect, especially of OBL, suggests a possible compensatory mechanism to limit the inflammatory reaction during infection.

Item Type: Article
MeSH: Animals ; Bone Marrow Cells/cytology ; Bone Marrow Cells/drug effects ; Bone Marrow Cells/metabolism ; Cell Differentiation ; Cells, Cultured ; Chemokine CCL2/biosynthesis ; Chemokine CCL5/biosynthesis ; Chemokine CXCL10 ; Chemokine CXCL13/biosynthesis ; Female ; Inflammation ; Interleukin-1/biosynthesis ; Interleukin-6/biosynthesis ; Lipopolysaccharides/immunology ; Lymphocyte Activation ; Lymphocytes/immunology ; Lymphocytes/metabolism ; Mice ; Mice, Inbred C57BL ; Osteoblasts/immunology ; Osteoblasts/metabolism ; Osteoclasts/immunology ; Osteoclasts/metabolism ; Osteoprotegerin/biosynthesis ; RANK Ligand/biosynthesis ; RANK Ligand/metabolism ; Tumor Necrosis Factor-alpha/biosynthesis ; Up-Regulation
Departments: Katedra za anatomiju i kliničku anatomiju
Katedra za fiziologiju i imunologiju
Depositing User: Marijan Šember
Status: Published
Kovačić, NatašaUNSPECIFIED
Katavić, VedranUNSPECIFIED
Aguila, Hector LeonardoUNSPECIFIED
Date: October 2012
Date Deposited: 21 Jan 2016 11:51
Last Modified: 08 Jul 2020 09:52
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