Antinociceptive effect of botulinum toxin type A on experimental abdominal pain

Drinovac, Višnja and Bach-Rojecky, Lidija and Babić, Ana and Lacković, Zdravko (2014) Antinociceptive effect of botulinum toxin type A on experimental abdominal pain. European Journal of Pharmacology, 745. pp. 190-5. ISSN 0014-2999

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Abstract

Visceral pain, especially in the abdominal region, represents one of the most common types of pain. Its chronic form is usually very hard to treat by conventional analgesic agents and adjuvants. We investigated the antinociceptive effect of botulinum toxin type A (BTX-A) in male Wistar rats in two models of visceral pain: peritonitis induced by intraperitoneal injection of 1% acetic acid and colitis induced by intracolonic instillation of 0.1% capsaicin. Pain was measured as the number of abdominal writhes. Additionally, referred mechanical sensitivity in the ventral abdominal area was evaluated by von Frey test and the extent of spinal c-Fos expression was immunohistochemically examined. BTX-A significantly reduced the number of abdominal writhes in both models of visceral pain after intrathecal application in a dose of 2 U/kg. In the experimental colitis model, BTX-A (2 U/kg) reduced both referred mechanical allodynia and c-Fos expression in the dorsal horn of the spinal cord (S2/S3 segments). In contrast to intrathecal administration, BTX-A (2 U/kg) administered into the cisterna magna had no effect on pain suggesting that the primary site of its action is a spinal cord.

Item Type: Article
MeSH: Abdominal Pain/drug therapy ; Abdominal Pain/etiology ; Abdominal Pain/physiopathology ; Acetic Acid/toxicity ; Analgesics/administration & dosage ; Analgesics/therapeutic use ; Animals ; Botulinum Toxins, Type A/administration & dosage ; Botulinum Toxins, Type A/therapeutic use ; Capsaicin/toxicity ; Colitis/complications ; Colitis/physiopathology ; Disease Models, Animal ; Hyperalgesia/drug therapy ; Hyperalgesia/etiology ; Hyperalgesia/physiopathology ; Male ; Pain Measurement ; Pain, Referred/drug therapy ; Pain, Referred/etiology ; Pain, Referred/physiopathology ; Peritonitis/complications ; Peritonitis/physiopathology ; Proto-Oncogene Proteins c-fos/metabolism ; Rats ; Rats, Wistar ; Spinal Cord/drug effects ; Spinal Cord/physiopathology ; Visceral Pain/drug therapy ; Visceral Pain/etiology ; Visceral Pain/physiopathology
Departments: Katedra za farmakologiju
Depositing User: Marijan Šember
Status: Published
Creators:
CreatorsEmail
Drinovac, VišnjaUNSPECIFIED
Bach-Rojecky, LidijaUNSPECIFIED
Babić, AnaUNSPECIFIED
Lacković, ZdravkoUNSPECIFIED
Date: 15 December 2014
Date Deposited: 15 Sep 2015 15:56
Last Modified: 15 Sep 2015 15:56
Subjects: /
Related URLs:
URI: http://medlib.mef.hr/id/eprint/2259

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