Pseudocholinesterase activity in cerebrospinal fluid as a biomarker of solid central nervous system tumors in children

Mikecin, Lili and Križmarić, Miljenko and Stepan Giljević, Jasminka and Gjurašin, Miroslav and Kern, Josipa and Leniček Krleža, Jasna and Popović, Ljiljana (2013) Pseudocholinesterase activity in cerebrospinal fluid as a biomarker of solid central nervous system tumors in children. Croatian Medical Journal, 54 (5). pp. 429-435. ISSN 0353-9504

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Abstract

AIM: To determine the activity of pseudocholinesterase (PChE) in cerebrospinal fluid (CSF) and serum in children with solid central nervous system (CNS) tumor and to assess whether PChE activity could be a valid biomarker for solid CNS tumors in children. ----- METHODS: The study and control group included 30 children each. Children in the study group had a solid CNS tumor, while those from the control group had never suffered from any tumor diseases. CSF and serum samples were collected from all participants and PChE activity was determined using the Ellman's spectrophotometric method. PChE activity in CSF was shown as a cerebrospinal fluid/serum ratio expressed in percentage, ie, PChE CSF/serum ratio. Receiver operating characteristic (ROC) curve was used to assess whether PChE activity can be used as a biomarker for identifying children with solid CNS tumors. ----- RESULTS: Children with solid CNS tumor had significantly higher PChE activity in CSF and serum, as well as PChE CSF/serum ratio (P=0.001). PChE CSF/serum ratio in the study group was 2.38% (interquartile range [IQR] 1.14-3.97) and 1.09% (IQR 0.95-1.45) in the control group. ROC curve analysis of PChE CSF/serum ratio resulted in an area under the curve (AUC) value of 0.76 (95% confidence interval [CI] 0.63-0.88) and a cut-off of 1.09. Twenty five of 29 patients with elevated PChE CSF/serum ratio had a tumor, corresponding to a sensitivity of 83% and a specificity of 53%. ----- CONCLUSION: PChE CSF/serum ratio may be used as a test or biomarker with good sensitivity for solid CNS tumors in children.

Item Type: Article
MeSH: Adolescent ; Case-Control Studies ; Central Nervous System Neoplasms/blood ; Central Nervous System Neoplasms/cerebrospinal fluid ; Central Nervous System Neoplasms/diagnosis ; Child ; Child, Preschool ; Female ; Humans ; Male ; Pseudocholinesterase/blood ; Pseudocholinesterase/cerebrospinal fluid ; ROC Curve ; Sensitivity and Specificity ; Tumor Markers, Biological/blood ; Tumor Markers, Biological/cerebrospinal fluid
Departments: Katedra za medicinsku statistiku, epidemiologiju i medicinsku informatiku
Depositing User: Marijan Šember
Status: Published
Creators:
CreatorsEmail
Mikecin, LiliUNSPECIFIED
Križmarić, MiljenkoUNSPECIFIED
Stepan Giljević, JasminkaUNSPECIFIED
Gjurašin, MiroslavUNSPECIFIED
Kern, JosipaUNSPECIFIED
Leniček Krleža, JasnaUNSPECIFIED
Popović, LjiljanaUNSPECIFIED
Date: 28 October 2013
Date Deposited: 19 Sep 2014 10:29
Last Modified: 13 Jul 2020 11:22
Subjects: /
Related URLs:
URI: http://medlib.mef.hr/id/eprint/2137

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