Efficacy of ozone on microorganisms in the tooth root canal [Djelotvornost ozona na mikroorganizme u korijenskom kanalu zuba]

Halbauer, Kristina and Prskalo, Katica and Janković, Bernard and Tarle, Zrinka and Pandurić, Vlatko and Kalenić, Smilja (2013) Efficacy of ozone on microorganisms in the tooth root canal [Djelotvornost ozona na mikroorganizme u korijenskom kanalu zuba]. Collegium Antropologicum, 37 (1). pp. 101-107. ISSN 0350-6134

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Abstract

The aim of this study was to examine the effect of ozone gas on the remaining bacteria after chemomechanical instrumentation of tooth root canal. The study was carried out at the Department of Endodontics and Restorative dentistry, School of Dental Medicine, University of Zagreb. A total of 37 tooth root canals from 23 teeth (10 incisors, 2 canines, 8 premolars and 3 molars) with a diagnosis of chronic apical periodontitis (17 untreated teeth and 6 retreatments) from 20 adult patients (11 females and 9 male) were selected. Endodontic samples consisted of 74 swabs from 37 canals. The first root canal swab was taken following a completed chemomechanical instrumentation by a sterile paper point after rinsing the root canal with a sterile saline solution. The canal was dried and treated with ozone gas for 40 seconds (HealOzone, Kavo, Germany). After the ozone treatment the canal was rinsed with a sterile saline solution a second swab was taken. The swabs were stored in transport media until cultivation. Microbiological identification was performed by macromorphological, micromorphological, commercial biochemical test microbiological analysis and bacteria count. A significant decrease in the number of bacteria (p < 0.001) was found after the ozone treatment: the total number of bacteria was 82%, 67% of aerobic and 93% of anaerobic bacteria. When analysing individually, a significant decrease was found for Streptococcus mitis and Propionibacterium acnes (p < 0.05). The results of this study shows the efficacy of ozone on the bacterial count reduction in the root canal treatment.

Abstract in Croatian

Svrha istraživanja bila je ispitati djelovanje ozona na bakterije preostale nakon kemijsko-mehaničke instrumentacije korijenskog kanala zuba. Istraživanje je provedeno na Zavodu za endodonciju i restorativnu stomatologiju Stomatološkog fakulteta Sveučilišta u Zagrebu. Sudjelovalo je 11 žena i 9 muškaraca upućenih na endodontsko liječenje. Dijagnozu neliječenog kroničnog apikalnog parodontitisa imalo je 17 zuba a kod 6 je bila potrebna revizija endodontskog liječenja. Ispitivani uzorak činila su 74 brisa iz 37 korijenskih kanala 23 zuba (10 sjekutića, 2 očnjaka, 8 pretkutnjaka, 3 kutnjaka). Prvi bris korijenskog kanala uzet je nakon završene kemijsko-mehaničke instrumentacije sterilnim papirnatim štapićem nakon ispiranja korijenskog kanala sterilnom fiziološkom otopinom. Posušeni kanal je tretiran ozonom 40 sekunda (HealOzone, Kavo, Njemačka). Nakon primjene ozona kanal je ispran sterilnom fiziološkom otopinom i uzet je drugi bris kanala. Brisovi su pohranjeni u transportne medije do kultivacije. Mikrobiološka analiza rađena je makromorfološkom, mikromorfološkom metodom i identifikacijom komercijalnim biokemijskim testovima. Analizom rezultata utvrđeno je statistički značajno smanjenje broja bakterija (p<0,001) nakon primjene ozona: ukupnog broja bakterija 82%, aerobnih 67%, anaerobnih 93%. Pojedinačnom analizom bakterija, značajno smanjenje nađeno je za Streptococcus mitis i Propionibacterium acnes (p<0,05). Ovo istraživanje je pokazalo djelotvornost ozona na smanjenje broja bakterija zaostalih nakon kemijsko-mehaničke instrumentacije korijenskog kanala.

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