Carboplatin resistant human laryngeal carcinoma cells are cross resistant to curcumin due to reduced curcumin accumulation

Rak, Sanjica and Čimbora-Zovko, Tamara and Gajski, Goran and Dubravčić, Klara and Domijan, Ana-Marija and Delaš, Ivančica and Garaj-Vrhovac, Verica and Batinić, Drago and Sorić, Jasna and Osmak, Maja (2013) Carboplatin resistant human laryngeal carcinoma cells are cross resistant to curcumin due to reduced curcumin accumulation. Toxicology in Vitro, 27 (2). pp. 523-532. ISSN 0887-2333

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Curcumin is a natural compound that exhibits a wide range of beneficial effects, among them the anti-tumor activity. Recently it was shown that curcumin may be efficient against drug resistant tumor cells. The goal of our investigation was to examine if human laryngeal carcinoma cells resistant to carboplatin display sensitivity to curcumin, as compared to parental cells, and if this sensitivity is altered, to determine the molecular mechanisms that are responsible for it. We found that carboplatin resistant 7T cells were also cross resistant to curcumin. After the treatment with equimolar concentration of curcumin, 7T cells exhibited lower intracellular accumulation of curcumin which coincided with reduced formation of reactive oxygen species (ROS), diminished lipid and DNA damage followed by reduced induction of apoptosis and expression of heat shock protein 70 (Hsp70), as compared to parental HEp-2 cells. However, after the treatment with equitoxic concentration of curcumin, intracellular accumulation and all the explored downstream effects were similar in both cell lines suggesting that resistance of 7T cells to curcumin was based on its reduced intracellular accumulation. Since curcumin accumulates mostly in the membranes, we explored the fatty acid composition of both cell lines, but we did not find any difference between them.

Item Type: Article
MeSH: Antineoplastic Agents / pharmacology ; Apoptosis ; Carboplatin / pharmacology ; Carcinoma / drug therapy ; Carcinoma / metabolism ; Cell Line, Tumor ; Cell Survival / drug effects ; Curcumin / pharmacology ; DNA Damage ; Drug Resistance, Multiple ; Drug Resistance, Neoplasm ; Fatty Acids / metabolism ; HSP70 Heat-Shock Proteins / metabolism ; Humans ; Laryngeal Neoplasms / drug therapy ; Laryngeal Neoplasms / metabolism ; Lipid Peroxidation / drug effects ; Reactive Oxygen Species / metabolism
Departments: Katedra za fiziologiju i imunologiju
Katedra za medicinsku kemiju, biokemiju i kliničku kemiju
Depositing User: Marijan Šember
Status: Published
Čimbora-Zovko, TamaraUNSPECIFIED
Dubravčić, KlaraUNSPECIFIED
Domijan, Ana-MarijaUNSPECIFIED
Delaš, IvančicaUNSPECIFIED
Garaj-Vrhovac, VericaUNSPECIFIED
Date: March 2013
Date Deposited: 13 Feb 2013 11:35
Last Modified: 14 Jul 2020 11:30
Subjects: /
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