Izraženost glipikana-3, beta-katenina i CD34 u hepatocelularnom karcinomu bolesnika s transplantiranom jetrom [Expression of glypican-3, beta-catenin and CD34 in hepatocellular carcinoma in patients with orthotopic liver transplantation]

Lovrić, Eva (2012) Izraženost glipikana-3, beta-katenina i CD34 u hepatocelularnom karcinomu bolesnika s transplantiranom jetrom [Expression of glypican-3, beta-catenin and CD34 in hepatocellular carcinoma in patients with orthotopic liver transplantation]. PhD thesis, Sveučilište u Zagrebu.

Preview

PDF Download (845kB) | Preview

Abstract

The aim of the study was to determine morphological features of HCC, clinical and laboratory data and characteristics of immunohistochemical expression of glipican-3 (GPC3), beta - catenin and CD34 in HCC, their mutual association and prognostic value, with special regard to those patient groups meeting and those exceeding milan criteria. 71 patients with primary HCC have undergone liver transplantation. Pathological tumor characteristics - number and size of tumors, architectural pattern, histological type, differentiation grade, and macro and microvascular invasion were analyzed. 30 patients meeting and 41 exceeding milan criteria. Vascular invasion was present in 14 patients. Cytoplasmatic GPC3 positivity was observed in 67.60% cases of HCC. Membrane beta-catenin was expressed in all cases of HCCs. The strong expression of CD34 was observed in all cases of HCCs. There was no significant difference between group meeting and exceeding milan criteria according to expression's characteristics of GPC3, beta-catenin and CD34. There was no difference in survival rate and recurrence between patient groups meeting and exceeding milan criteria. Vascular invasion was more frequently observed in patients group with shorter period of recurrence. Membranous, strong intensity of beta-catenin was more frequently observed in patients group with longer survival rate.

Abstract in Croatian

Cilj istraživanja je bio odrediti morfološke karakteristike hepatocelularnog karcinoma (HCK-a), kliničke i laboratorijske pokazatelje i imunohistokemijske karakteristike izraženosti glipikana-3 (GPC3), beta-katenina i CD34 u HCK-u, njihovu međusobnu povezanost te utjecaj na ishod bolesnika unutar i izvan milanskih kriterija. 71 bolesnik sa primarnim HCK-om, liječen je transplantacijom jetre. Analizirane su morfološke karakteristike tumora - broj i veličina, arhitekturalni tip rasta, histološki tip, stupanj diferencijacije, prisutnost makro i mikrožilne invazije. 30 bolesnika je bilo unutar milanskih kriterija i 41 bolesnik izvan milanskih kriterija. Kod 14 bolesnika je bila prisutna žilna invazija tumora. Pozitivna izraženost GPC3 je bila prisutna u 67.60% slučajeva HCK-a, citoplazmatske lokalizacije. Pozitivna izraženost beta-katenina bila je prisutna u svim HCK-a, membranske lokalizacije. Izraženost CD34 u svim slučajevima bila je jakog intenziteta. Karakteristike izraženosti biljega GPC3, beta-katenina i CD34 ne razlikuju se u skupinama bolesnika određenih milanskim kriterijima. Analizirane skupine bolesnika određene milanskim kriterijima nisu se razlikovale ukupnim preživljenjem, niti vremenom do ponovne pojave bolesti. Žilna invazija bila je značajno češće prisutna u skupini bolesnika s kraćim vremenom do ponovne pojave bolesti. Jaki intenzitet izraženosti beta-katenina bio je značajno češće prisutan u skupini bolesnika dužeg ukupnog preživljenja.

Actions (login required)

View Item

Downloads