Botulinum toxin's axonal transport from periphery to the spinal cord

Matak, Ivica and Riederer, Peter and Lacković, Zdravko (2012) Botulinum toxin's axonal transport from periphery to the spinal cord. Neurochemistry International, 61 (2). pp. 236-9. ISSN 0197-0186

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Abstract

Axonal transport of enzymatically active botulinum toxin A (BTX-A) from periphery to the CNS has been described in facial and trigeminal nerve, leading to cleavage of synaptosomal-associated protein 25 (SNAP-25) in central nuclei. Aim of present study was to examine the existence of axonal transport of peripherally applied BTX-A to spinal cord via sciatic nerve. We employed BTX-A-cleaved SNAP-25 immunohistochemistry of lumbar spinal cord after intramuscular and subcutaneous hind limb injections, and intraneural BTX-A sciatic nerve injections. Truncated SNAP-25 in ipsilateral spinal cord ventral horns and dorsal horns appeared after single peripheral BTX-A administrations, even at low intramuscular dose applied (5 U/kg). Cleaved SNAP-25 appearance in the spinal cord after BTX-A injection into the sciatic nerve was prevented by proximal intrasciatic injection of colchicine (5 mM, 2 μl). Cleaved SNAP-25 in ventral horn, using choline-acetyltransferase (ChAT) double labeling, was localized within cholinergic neurons. These results extend the recent findings on BTX-A retrograde axonal transport in facial and trigeminal nerve. Appearance of truncated SNAP-25 in spinal cord following low-dose peripheral BTX-A suggest that the axonal transport of BTX-A occurs commonly following peripheral application.

Item Type: Article
MeSH: Animals ; Axonal Transport/physiology ; Botulinum Toxins, Type A/administration & dosage ; Botulinum Toxins, Type A/metabolism ; Choline O-Acetyltransferase/metabolism ; Colchicine/pharmacology ; Hindlimb/physiology ; Immunohistochemistry ; Injections ; Injections, Intramuscular ; Injections, Subcutaneous ; Male ; Microscopy, Confocal ; Motor Neurons/metabolism ; Peripheral Nerves/metabolism ; Rats ; Rats, Wistar ; Sciatic Nerve/metabolism ; Sensory Receptor Cells/metabolism ; Spinal Cord/metabolism ; Synaptosomal-Associated Protein 25/metabolism
Departments: Katedra za farmakologiju
Depositing User: Marijan Šember
Status: Published
Creators:
CreatorsEmail
Matak, IvicaUNSPECIFIED
Riederer, PeterUNSPECIFIED
Lacković, ZdravkoUNSPECIFIED
Date: July 2012
Date Deposited: 07 Jan 2013 10:24
Last Modified: 07 Jan 2013 10:24
Subjects: /
Related URLs:
URI: http://medlib.mef.hr/id/eprint/1727

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