Koštani fenotip miševa s transgenom za interleukin 7 (IL-7) [Bone phenotype of IL-7 transgenic mice]

Žitnjak, Daniela (2009) Koštani fenotip miševa s transgenom za interleukin 7 (IL-7) [Bone phenotype of IL-7 transgenic mice]. PhD thesis, Sveučilište u Zagrebu.

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Abstract

Mice with an interleukin (IL)-7 transgene under the control of Eα promoter overexpress IL-7 in MHC class II-positive cells and develop specific immune phenotype, marked by an increase in CD45R+ cells in both the bone marrow and peripheral blood. We show that female IL-7 transgenic mice have a bone phenotype characterized by an age-related loss of trabecular bone in both axial and long bones. Osteopenia was the result of increased number of active osteoclasts on the surface of trabecular bone. Furthermore, IL-7 transgenic mice showed increased osteoclastic but unchanged osteoblastic potential of the bone marrow in vitro, both from nonatherent bone marrow cells and CD45R+ marrow cells. IL-7 over-expression also created osteoclastogenic microenvironment within the bone marrow which promoted the commitment of precursors towards the osteoclast lineage.

Abstract in Croatian

Miševi s transgenom za IL-7 koji je pod kontrolom Eα promotora pojačano izražavaju IL-7 u svim stanicama koje na staničnoj membrane nose MHC II antigen te razvijaju karakterističan imunološki fenotip koji obilježava porast udjela stanica pozitivnih za CD45R antigen u koštanoj srži i u perifernoj krvi. Ženke tih miševa pokazuju i koštani fenotip obilježen gubitkom trabekularne kosti u aksijalnim i dugim kostima koji se razvija tijekom njihovog starenja. Osteopenija je posljedica povećanog broja aktivnih osteoklasta na površini trabekularne kosti. Nadalje, u kulturama neadheriranih i CD45R+ stanica koštane srži transgeničnih miševa nisu uočene promjene u broju osteoblasta, unatoč povećanom osteoklastogeničnom potencijalu koštane srži. Povećani izražaj IL-7 dovodi do promjena u mikrookolišu unutar koštane srži čime su stanice preteče usmjerene prema osteoklastnoj lozi.

Item Type: Thesis (PhD)
Mentors:
Mentor
Marušić, Ana
Departments: Izvan medicinskog fakulteta
Depositing User: dr.med. Helena Markulin
University: Sveučilište u Zagrebu
Institution: Medicinski fakultet
Number of Pages: 105
Status: Unpublished
Creators:
CreatorsEmail
Žitnjak, DanielaUNSPECIFIED
Date: 3 November 2009
Date Deposited: 26 Oct 2012 12:12
Last Modified: 29 Oct 2012 14:54
Subjects: WD Disorders of Systemic, Metabolic or Environmental Origin, etc. > WD 200-226 Metabolic Diseases > WD 200-200.1 General Metabolic Diseases
Related URLs:
    URI: http://medlib.mef.hr/id/eprint/1645

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