Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

In vivo fate mapping identifies mesenchymal progenitor cells

Grčević, Danka and Pejda, Slavica and Matthews, Brya G. and Repić, Dario and Wang, Liping and Li, Haitao and Kronenberg, Mark S. and Jiang, Xi and Maye, Peter and Adams, Douglas J. and Rowe, David W. and Aguila, Hector L. and Kalajzić, Ivo (2012) In vivo fate mapping identifies mesenchymal progenitor cells. Stem Cells, 30 (2). pp. 187-196. ISSN 1066-5099

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    English abstract

    Adult mesenchymal progenitor cells have enormous potential for use in regenerative medicine. However, the true identity of the progenitors in vivo and their progeny has not been precisely defined. We hypothesize that cells expressing a smooth muscle α-actin promoter (αSMA)-directed Cre transgene represent mesenchymal progenitors of adult bone tissue. By combining complementary colors in combination with transgenes activating at mature stages of the lineage, we characterized the phenotype and confirmed the ability of isolated αSMA(+) cells to progress from a progenitor to fully mature state. In vivo lineage tracing experiments using a new bone formation model confirmed the osteogenic phenotype of αSMA(+) cells. In vitro analysis of the in vivo-labeled SMA9(+) cells supported their differentiation potential into mesenchymal lineages. Using a fracture-healing model, αSMA9(+) cells served as a pool of fibrocartilage and skeletal progenitors. Confirmation of the transition of αSMA9(+) progenitor cells to mature osteoblasts during fracture healing was assessed by activation of bone-specific Col2.3emd transgene. Our findings provide a novel in vivo identification of defined population of mesenchymal progenitor cells with active role in bone remodeling and regeneration.

    Item Type: Article
    MeSH: Actins/genetics ; Actins/metabolism ; Animals ; Antigens, Differentiation/metabolism ; Bone Marrow Cells/metabolism ; Bone Regeneration ; Bone Remodeling ; Cell Differentiation ; Cell Lineage ; Female ; Fracture Healing ; Gene Expression Regulation ; Green Fluorescent Proteins/biosynthesis ; Green Fluorescent Proteins/genetics ; Male ; Mesenchymal Stem Cells/metabolism ; Mice ; Mice, Transgenic ; Phenotype ; Promoter Regions, Genetic ; Recombinant Fusion Proteins/biosynthesis ; Recombinant Fusion Proteins/genetics ; Tibia/pathology
    Divisions: Katedra za fiziologiju i imunologiju
    Depositing User: Marijan Šember
    Status: Published
    Grčević, Danka
    Pejda, Slavica
    Matthews, Brya G.
    Repić, Dario
    Wang, Liping
    Li, Haitao
    Kronenberg, Mark S.
    Jiang, Xi
    Maye, Peter
    Adams, Douglas J.
    Rowe, David W.
    Aguila, Hector L.
    Kalajzić, Ivo
    Date: February 2012
    Date Deposited: 16 Oct 2012 15:41
    Last Modified: 16 Oct 2012 15:41
    Subjects: /
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