In vivo fate mapping identifies mesenchymal progenitor cells

Grčević, Danka and Pejda, Slavica and Matthews, Brya G. and Repić, Dario and Wang, Liping and Li, Haitao and Kronenberg, Mark S. and Jiang, Xi and Maye, Peter and Adams, Douglas J. and Rowe, David W. and Aguila, Hector L. and Kalajzić, Ivo (2012) In vivo fate mapping identifies mesenchymal progenitor cells. Stem Cells, 30 (2). pp. 187-196. ISSN 1066-5099

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Abstract

Adult mesenchymal progenitor cells have enormous potential for use in regenerative medicine. However, the true identity of the progenitors in vivo and their progeny has not been precisely defined. We hypothesize that cells expressing a smooth muscle α-actin promoter (αSMA)-directed Cre transgene represent mesenchymal progenitors of adult bone tissue. By combining complementary colors in combination with transgenes activating at mature stages of the lineage, we characterized the phenotype and confirmed the ability of isolated αSMA(+) cells to progress from a progenitor to fully mature state. In vivo lineage tracing experiments using a new bone formation model confirmed the osteogenic phenotype of αSMA(+) cells. In vitro analysis of the in vivo-labeled SMA9(+) cells supported their differentiation potential into mesenchymal lineages. Using a fracture-healing model, αSMA9(+) cells served as a pool of fibrocartilage and skeletal progenitors. Confirmation of the transition of αSMA9(+) progenitor cells to mature osteoblasts during fracture healing was assessed by activation of bone-specific Col2.3emd transgene. Our findings provide a novel in vivo identification of defined population of mesenchymal progenitor cells with active role in bone remodeling and regeneration.

Item Type: Article
MeSH: Actins/genetics ; Actins/metabolism ; Animals ; Antigens, Differentiation/metabolism ; Bone Marrow Cells/metabolism ; Bone Regeneration ; Bone Remodeling ; Cell Differentiation ; Cell Lineage ; Female ; Fracture Healing ; Gene Expression Regulation ; Green Fluorescent Proteins/biosynthesis ; Green Fluorescent Proteins/genetics ; Male ; Mesenchymal Stem Cells/metabolism ; Mice ; Mice, Transgenic ; Phenotype ; Promoter Regions, Genetic ; Recombinant Fusion Proteins/biosynthesis ; Recombinant Fusion Proteins/genetics ; Tibia/pathology
Departments: Katedra za fiziologiju i imunologiju
Depositing User: Marijan Šember
Status: Published
Creators:
CreatorsEmail
Grčević, DankaUNSPECIFIED
Pejda, SlavicaUNSPECIFIED
Matthews, Brya G.UNSPECIFIED
Repić, DarioUNSPECIFIED
Wang, LipingUNSPECIFIED
Li, HaitaoUNSPECIFIED
Kronenberg, Mark S.UNSPECIFIED
Jiang, XiUNSPECIFIED
Maye, PeterUNSPECIFIED
Adams, Douglas J.UNSPECIFIED
Rowe, David W.UNSPECIFIED
Aguila, Hector L.UNSPECIFIED
Kalajzić, IvoUNSPECIFIED
Date: February 2012
Date Deposited: 16 Oct 2012 13:41
Last Modified: 16 Oct 2012 13:41
Subjects: /
Related URLs:
URI: http://medlib.mef.hr/id/eprint/1638

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