Ekspresija autokrinog hormona rasta, receptora hormona rasta, plakoglobina i NEDD9 u duktalnom invazivnom karcinomu dojke s i bez metastaza

Štajduhar, Emil (2012) Ekspresija autokrinog hormona rasta, receptora hormona rasta, plakoglobina i NEDD9 u duktalnom invazivnom karcinomu dojke s i bez metastaza. PhD thesis, Sveučilište u Zagrebu.

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Abstract

Breast cancer is one of the leading causes of death in women. Mammographic screening for an early detection of breast cancer has been performed in Croatia for the last few years. The samples used in this study mostly belong to the patients that went to routine mammographic screening at the Clinical Hospital Center Sisters of Charity. The thesis hypothesis was: the expression of autocrine growth hormone (GH), its receptor (GHR), and NEED9 are increased, while the expression of plakoglobin expression is decreased in metastasing breast cancer. All analyzed samples were of G2 histologycal grade. Immunochistochemistry was performed for studying the expression of GH, GHR, NEDD9 and plakoglobin in both 40 non-metastatic, and 40 metastastatic breast cancer. Additionally, 40 lymph node metastases were analyzed in the same way. Study parameters were correlated with with ER, PR and HER2 expression. Age of patients and tumor size were analyzed, too. Experimental results have been analyzed with appropriate statistical methods. Plakoglobin and NEDD9 expression is significantly increased in epitelial tumor component of both tumor groups, also in epithelial component of metastases in lymph nodes. In the stromal component expression is decreased. GH expression is decreased in epithelial and stromal tumor component of both tumor groups, also of lymph node metastases. GHR expression is increased in stromal component of both tumor groups, also of metastases, while in epithelial component is very weak. GHR and NEDD9 antagonists may be of high clinical and therapeutic value by reducing chemotherapy side effects in breast cancer patients.

Abstract in Croatian

Karcinom dojke glavnik je uzrok smrti u žena. Probir mamografija, u svrhu ranog otkrivanja karcinoma dojke provodi se u Hrvatskoj zadnjih nekoliko godina. U ovom se istraživanju koriste uzorci karcinoma dojke otkriveni metodom probira u Kliničkom bolničkom centru „Sestre Milosrdnice“. Hipoteza istraživanja bila je: u metastatskom karcinomu dojke povišena je ekspresija autokrinog hormona rasta (HR), receptora hormona rasta (RHR) i NEDD9, a snižena je ekspresija plakoglobina. Cilj istraživanja bio je utvrditi ekspresiju RH, RHR, NEDD9 i plakoglobina u karcinomima dojke bez metastaza, karcinomima dojke s metastazama i u metastazama u limfnim čvorovima, metodom imunohistokemije. Svi analizirani uzorci bili su histološkog gradusa „G2“. Istraživani parametri uspoređivani su s ispoljenošću ER, PR and HER2, a analizirani su još i veličina tumora, kao i dob bolesnica. U analizu je bilo uključeno 40 karcinoma bez metastaza, 40 karcinoma s metastazama i 40 metastaza u limfnim čvorovima. Eksperimentalni rezultati studije analizirani su adekvatnim statističkim metodama. Ekspresija plakoglobina i NEDD9 u epitelnoj komponenti tumora značajno je povećana u obje skupine tumora, kao i u epitelnoj komponenti metastaza u limfnim čvorovima, dok je u stromalnoj komponenti izrazito slaba ili izostala. Ekspresija HR je izrazito slaba kako u epitelnoj, tako i u stromalnoj komponenti obje skupine tumora i metastaza u limfnim čvorovima. Ekspresija RHR je povećana u stromalnoj komponenti obje skupine tumora kao i metastaza, dok je u epitelnoj komponenti izrazito slaba. RHR i NEDD9 antagonisti bi mogli biti od značajnog kliničkog značenja i terapijske važnosti a štetni efekti kemoterapije bi se značajno umanjili.

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