The influence of tumor necrosis factor microsatellite polymorphisms on patient survival following hematopoietic stem cell transplantation

Štingl, Katarina and Žunec, Renata and Serventi-Seiwerth, Ranka and Labar, Boris and Grubić, Zorana (2012) The influence of tumor necrosis factor microsatellite polymorphisms on patient survival following hematopoietic stem cell transplantation. Croatian Medical Journal, 53 (1). pp. 24-29. ISSN 0353-9504

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Abstract

AIM: To investigate the influence of tumor necrosis factor (TNF) microsatellite polymorphisms on patient survival following hematopoietic stem cell transplantation. ----- METHODS: We analyzed TNFa, TNFb, and TNFd microsatellites among 100 patients who underwent allogeneic hematopoietic stem cell transplantation from a human leukocyte antigen (HLA)-identical sibling donor at the Internal Clinic of the University Hospital Center Zagreb in the period 2001-2009. The analysis was performed using polymerase chain reaction amplification and electrophoresis on a polyacrylamide gel in an automated sequencer. ----- RESULTS: There was no significant difference in patient survival with respect to the allele length at a given microsatellite. However, a significantly lower survival rate was noticed among patients who were positive for TNFa8 allele (P<0.001) and a significantly higher survival rate among those who were positive for TNFa10 allele (P=0.0220). ----- CONCLUSION: These results for the first time suggest an influence of TNFa microsatellite on patient survival following HSCT and indicate a need for further studies of this microsatellite.

Abstract in Croatian

Utjecaj mikrosatelitnih polimorfizama vezanih uz čimbenik nekroze tumora na preživljenje pacijenata nakon transplantacije matičnih hematopoetskih stanica ----- Cilj Istražiti utjecaj mikrosatelitnih polimorfizama vezanih uz čimbenik nekroze tumora (eng. TNF) na preživljenje pacijenata nakon transplantacije matičnih hematopoetskih stanica. ----- Metode Analizirali smo TNFa, TNFb i TNFd mikrosatelite kod 100 pacijenata koji su bili podvrgnuti alogenskoj transplantaciji matičnih hematopoetskih stanica od HLA-identičnog darivatelja koji im je brat ili sestra na Internoj klinici Kliničko-bolničkog centra Zagreb. Analiza je provedena uz pomoć amplifikacije lančanom reakcijom polimeraze i elektroforeze na poliakrilamidnom gelu u automatskom sekvenceru. ----- Rezultati Nije bilo značajne razlike u preživljenju pacijenata s obzirom na duljinu alela na određenom mikrosatelitu. Ipak značajno lošiju stopu preživljenja imali su pacijenti pozitivni na TNFa8 alel (P<0,001), dok su bolju stopu preživljenja imali pacijenti pozitivni na TNFa10 alel (P=0,0220). ----- Zaključak Ovi rezultati po prvi put ukazuju na utjecaj TNFa mikrosatelita na preživljenje pacijenata nakon transplantacije matičnih hematopoetskih stanica kao i na potrebu za daljnjim proučavanjem ovog mikrosatelita.

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