Expression of MAGE-A and NY-ESO-1 cancer/testis antigens in medullary breast cancer: retrospective immunohistochemical study

Matković, Božica and Juretić, Antonio and Spagnoli, Giulio C. and Šeparović, Viktor and Gamulin, Marija and Šeparović, Robert and Šarić, Nera and Bašić-Koretić, Martina and Novosel, Irena and Krušlin, Božo (2011) Expression of MAGE-A and NY-ESO-1 cancer/testis antigens in medullary breast cancer: retrospective immunohistochemical study. Croatian Medical Journal, 52 (2). pp. 171-177. ISSN 0353-9504

Download (938kB) | Preview


AIM: To immunohistochemically evaluate the expression of MAGE-A1, MAGE-A, and NY-ESO-1 cancer/testis (C/T) tumor antigens in medullary breast cancer (MBC) tumor samples and to analyze it in relation to the clinicopathological features. ----- METHODS: This retrospective study included samples from 49 patients: 40 with typical MBC and 9 with atypical MBC. Tumor specimens were obtained from patients operated on in the University Hospital for Tumors and the Sisters of Mercy University Hospital, Zagreb, Croatia, from 1999 to 2005. Standard immunohistochemistry was used on archival paraffin-embedded MBC tissues. ----- RESULTS: MAGE-A1, MAGE-A, and NY-ESO-1 antigens were expressed in 33% (16/49), 33% (16/49), and 22% (11/49) of patients, respectively. No difference between the groups with and without C/T tumor antigen expression in age at diagnosis, tumor size, axillary lymph node metastasis, adjuvant therapy, and HER-2 expression was identified. Significantly more patients died in the MAGE-A-positive group than in the MAGE-A-negative group (P=0.010), whereas a borderline significance was found between MAGE-A1-positive and the MAGE-A1-negative group (P=0.079) and between NY-ESO-1-positive and NY-ESO-1-negative group (P=0.117). Overall survival, as evaluated by the Kaplan-Meier curves, was lower in MAGE-A1- (P=0.031), MAGE-A- (P=0.004), NY-ESO-1-positive groups (P=0.077). ----- CONCLUSION: Expression of C/T antigens may represent a marker of potential prognostic relevance in MBC.

Item Type: Article
MeSH: Adult ; Aged ; Aged, 80 and over ; Antigens/genetics ; Antigens/immunology ; Antigens, Neoplasm/analysis ; Antigens, Neoplasm/genetics ; Antigens, Neoplasm/immunology ; Breast Neoplasms/immunology ; Carcinoma, Medullary/immunology ; Croatia ; Female ; Gene Expression Regulation, Neoplastic/immunology ; Humans ; Immunohistochemistry ; Membrane Proteins/analysis ; Membrane Proteins/genetics ; Membrane Proteins/immunology ; Middle Aged ; Neoplasm Proteins/analysis ; Neoplasm Proteins/genetics ; Neoplasm Proteins/immunology ; Peptide Fragments/analysis ; Peptide Fragments/genetics ; Peptide Fragments/immunology ; Retrospective Studies ; Survival Analysis
Departments: Katedra za patologiju
Katedra za radiologiju i opću kliničku onkologiju
Depositing User: Marijan Šember
Status: Published
Matković, BožicaUNSPECIFIED
Juretić, AntonioUNSPECIFIED
Spagnoli, Giulio C.UNSPECIFIED
Šeparović, ViktorUNSPECIFIED
Gamulin, MarijaUNSPECIFIED
Šeparović, RobertUNSPECIFIED
Bašić-Koretić, MartinaUNSPECIFIED
Date: 15 April 2011
Date Deposited: 23 Jan 2012 11:07
Last Modified: 23 Jan 2012 11:07
Subjects: /
Related URLs:

Actions (login required)

View Item View Item


Downloads per month over past year