Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Pilot study of the association between the HLA region and testicular carcinoma among Croatian patients

Gotovac, Kristina and Grubić, Zorana and Kaštelan, Željko and Štingl, Katarina and Kuliš, Tomislav and Krhen, Ivan and Hudolin, Tvrtko and Kaštelan, Maja and Žunec, Renata (2011) Pilot study of the association between the HLA region and testicular carcinoma among Croatian patients. Urologia Internationalis, 87 (3). pp. 288-292. ISSN 0042-1138

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    English abstract

    OBJECTIVES: To analyze the distribution of HLA alleles and HLA microsatellite alleles in Croatian patients with testicular carcinoma, compare it with that of healthy controls and investigate whether the polymorphism within the HLA region could be associated with the development of testicular cancer. ----- METHODS: Genomic DNA was isolated from the peripheral blood of 24 patients with testicular germ cell tumors (TGCT). Patients and controls were typed for HLA class I and class II polymorphism by the PCR-SSO method. Nine HLA microsatellites were analyzed by PCR and electrophoresis in an automated sequencer. ----- RESULTS: No significant deviation in the distribution of frequencies at HLA class I alleles was observed between patients and controls. Among HLA class II alleles, a statistically significant increase in the frequency of the HLA-DPB1*1701 allele was found among patients. The frequency of the HLA-DRB1*07-DQA1*0201-DQB1*0202 haplotype was increased in patients in comparison to the controls. Analysis of HLA microsatellites showed an increased frequency of D6S291-3 allele (p(corr) = 0.0455, OR = 3.05) among patients. ----- CONCLUSIONS: The observed association of the disease and the DPB1*1701 allele as well as with the D6S291-3 allele suggests that this part of the HLA region might be involved in the pathogenesis of TGCT. Our data provide a basis for further studies about the correlation between the HLA region and testicular cancer.

    Item Type: Article
    Divisions: Katedra za urologiju
    Depositing User: Marijan Šember
    Status: Published
    Creators:
    CreatorsEmail
    Gotovac, Kristina
    Grubić, Zorana
    Kaštelan, Željko
    Štingl, Katarina
    Kuliš, Tomislav
    Krhen, Ivan
    Hudolin, Tvrtko
    Kaštelan, Maja
    Žunec, Renata
    Date: 2011
    Date Deposited: 09 Jan 2012 14:10
    Last Modified: 09 Jan 2012 14:10
    Subjects: /
    Related URLs:
    URI: http://medlib.mef.hr/id/eprint/1420

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