Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia

Bošnjak, Zrinka and Bedenić, Branka and Mazzariol, Annarita and Jarža-Davila, Neda and Šuto, Sandra and Kalenić, Smilja (2010) VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia. Scandinavian Journal of Infectious Diseases, 42 (3). pp. 193-197. ISSN 0036-5548

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    English abstract

    The aim of this investigation was to characterize metallo-beta-lactamases (MBLs) in Pseudomonas aeruginosa isolates from Zagreb, Croatia. One hundred P. aeruginosa isolates with reduced susceptibility to either imipenem or meropenem were tested for the production of MBLs by MBL-Etest. The susceptibility to a wide range of antibiotics was determined by broth microdilution method. The presence of bla(MBL) genes was detected by polymerase chain reaction (PCR). Hydrolysis of 0.1 mM imipenem by crude enzyme preparations of beta-lactamases was monitored by UV spectrophotometer. Outer membrane proteins were prepared and analysed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Six out of 100 isolates were positive for MBLs by Etest. All strains were resistant to gentamicin, ceftazidime and cefotaxime, and all except 1 were resistant to imipenem. Six strains positive for MBLs by Etest were identified as VIM MBL-producers by PCR. Sequencing of bla(VIM) genes revealed the production of VIM-2 beta-lactamase in all 6 strains. This investigation proved the occurrence of VIM-2 beta-lactamase among P. aeruginosa strains from Zagreb, Croatia. VIM-2 beta-lactamase with similar properties has previously been described in another region of Croatia and in Italy, France, Spain, Greece, Taiwan and South Korea, suggesting that this type of enzyme is widespread in the Mediterranean region of Europe and in the Far East.

    Item Type: Article
    MeSH: Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/biosynthesis ; Bacterial Proteins/genetics ; Croatia ; DNA, Bacterial/genetics ; Humans ; Hydrolysis ; Imipenem/metabolism ; Imipenem/pharmacology ; Microbial Sensitivity Tests ; Polymerase Chain Reaction ; Pseudomonas Infections/microbiology ; Pseudomonas aeruginosa/enzymology ; Pseudomonas aeruginosa/isolation & purification ; Sequence Analysis, DNA ; Spectrophotometry ; Thienamycins/pharmacology ; beta-Lactam Resistance ; beta-Lactamases/biosynthesis ; beta-Lactamases/genetics
    Divisions: Katedra za medicinsku mikrobiologiju i parazitologiju
    Depositing User: Marijan Šember
    Status: Published
    Bošnjak, Zrinka
    Bedenić, Branka
    Mazzariol, Annarita
    Jarža-Davila, Neda
    Šuto, Sandra
    Kalenić, Smilja
    Date: March 2010
    Date Deposited: 01 Dec 2011 14:02
    Last Modified: 01 Dec 2011 14:02
    Subjects: /
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