Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

C-myc expression in the microvessels of medulloblastoma

Stanić, Gabrijela and Čupić, Hrvoje and Žarković, Kamelija and Tomas, Davor and Krušlin, Božo (2011) C-myc expression in the microvessels of medulloblastoma. Collegium Antropologicum, 35 (1). pp. 39-42. ISSN 0350-6134

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    Croatian abstract

    EKSPRESIJA C-MYC-a U KAPILARAMA MEDULOBLASTOMA ----- Povećana ekspresija c-myc-a povezana je s neoplastičnom transformacijom i stvaranjem krvnih žila. Procjena ekspresije c-myc-a u endotelnim stanicama i neovaskularizacija mogu pomoći u procjeni biološkog ponašanja tumora. U našem radu analizirali smo neovaskularizaciju i ekspresiju c-myc-a u 36 uzoraka tkiva meduloblastoma. Rezultati su prikazani semikvantitativno, određivanjem imunohistokemijskog indeksa bojenja (IIB) koji je dobiven kao zbroj intenziteta reakcije (IR) i postotka pozitivnih stanica (PPS). Broj tumorskih krvnih žila (MVC-microvessel count) dobiven je na 10 najaktivnih područja neovaskularizacije pri povećanju od 400x. Brojčana vrijednost izražena je kao gustoća krvnih žila po mm2 (MVD-microvessel density). Imunohistoelimjski indeks bojanja za c-myc u endotelu krvnih žila grupirali smo u četri grupe, 0 – bez reakcije, I- slaba reakcija (IIB=1 ili 2), II – umjerena reakcija (IIB=3 ili 4), III – jaka reakcija (IIB=5 ili 6). Statistički značajne razlike (p=0,0214) našli smo između skupina 0 i 1 u odnosu na skupinu 2 i 3. Kod pacijenata muškog spola našli smo veći postotak reaktivnih stanica nego u pacijenata ženskog spola (p=0,0483). C-myc PPS 0 ili 1 ima u prosjeku manju gustoću krvnih žila po mm2 nego c-myc PPS 2 ili 3, ali razlika nije statistički značajna. C-myc IIB 0 ili 1 ima u prosjeku manju gustoću krvnih žila po mm2 nego c-myc IIB 2 ili 3, ali razlika nije statistički značajna. Na osnovu rezultata zaključili smo da je intenzitet c-myc bojanja povezan s većom gustoćom krvnih žila.

    English abstract

    The increased expression of c-myc is related to neoplastic transformation and angiogenesis. Therefore, the assessment of expression of c-myc in endothelial cells and neovascularization could help to determine the biological behavior of the tumor. We analyzed neovascularization and c-myc expression in 36 medulloblastoma specimens. The results were shown by determining immunohistochemical staining index (ISI), the sum of staining intensity (SI) and the percentage of positive cells (PPC) in the blood vessels endothelium of the tumor. We also performed the microvessel count (MVC) in 10 high-power fields (400X) with the most prominent vascularization and expressed it as microvessel density per mm2 (MVD). C-myc immunostaining intensity index in blood vessel endothelium is grouped into four groups, 0--no reaction, I-weak reaction (ISI = 1 or 2), II--moderate reaction (ISI = 3 or 4), III--strong reaction (ISI = 5 or 6). Statistically significant differences (p = 0.0214) have been found between groups 0 and 1 compared to groups 2 and 3. A higher percentage of positive cells has been found in male patients than in female ones (p = 0.0483). C-myc PPC 0 or 1 has on the average smaller density of blood vessels per mm2 than c-myc PPC 2 or 3, but the difference is not statistically significant. C-myc ISI 0 or 1 has, on the average, smaller density of blood vessels per mm2 than c-myc ISI 2 or 3, but the difference is not statistically significant. We concluded that c-myc staining intensity was associated with higher microvessels density.

    Item Type: Article
    MeSH: Endothelium, Vascular/metabolism ; Female ; Humans ; Male ; Medulloblastoma/blood supply ; Medulloblastoma/metabolism ; Microvessels/metabolism ; Neovascularization, Pathologic/metabolism ; Proto-Oncogene Proteins c-myc/biosynthesis
    Divisions: Katedra za patologiju
    Depositing User: Marijan Šember
    Status: Published
    Creators:
    CreatorsEmail
    Stanić, Gabrijela
    Čupić, Hrvoje
    Žarković, Kamelija
    Tomas, Davor
    Krušlin, Božo
    Date: March 2011
    Date Deposited: 30 Nov 2011 09:51
    Last Modified: 30 Nov 2011 09:51
    Subjects: /
    Related URLs:
    URI: http://medlib.mef.hr/id/eprint/1400

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