Endosomal location of dopamine receptors in neuronal cell cytoplasm

Wolstencroft, Elizabeth C. and Šimić, Goran and thi Man, Nguyen and Holt, Ian and Lam, Le Thanh and Buckland, Paul R. and Morris, Glenn E. (2007) Endosomal location of dopamine receptors in neuronal cell cytoplasm. Journal of Molecular Histology, 38 (4). pp. 333-340. ISSN 1567-2379 (Print) 1567-2387 (Electronic)

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English abstract

Five subtypes of dopamine receptor exist in two subfamilies: two D(1)-like (D(1) and D(5)) and three D(2)-like (D(2), D(3) and D(4)). We produced novel monoclonal antibodies against all three D(2)-like receptors and used them to localize receptors in Ntera-2 (NT-2) cells, the human neuronal precursor cell line. Most of the immunostaining for all three receptors colocalized with mannose-6-phosphate receptor, a marker for late endosomes formed by internalization of the plasma membrane. This result was obtained with antibodies against three different epitopes on the D(3) receptor, to rule out the possibility of cross-reaction with another protein, and controls without primary antibody or in the presence of competitor antigen were completely negative. In rat cerebral cortex and hippocampus, some of the dopamine receptor staining was found in similar structures in neuronal cell cytoplasm. Only some of the neurons were positive for dopamine receptors and the pattern was consistent with previously-reported patterns of innervation by dopamine-producing neurons. Endosomal dopamine receptors may provide a useful method for identifying cell bodies of dopamine-responsive neurons to complement methods that detect only active receptors in the neuronal cell membrane.

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• Endosomal location of dopamine receptors in neuronal cell cytoplasm. (deposited 24 Jan 2008)[Currently Displayed]
  • Contribution of consanguinuity to polygenic and multifactorial diseases. (deposited 29 Jan 2008)

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